Epigenetics

Epigenetics is the study of chemical modifications or physical structure changes to chromatin in the absence of DNA sequence alterations. These modifications modulate gene expression to enable cell differentiation and identity. Disruption of the epigenome from environmental factors can have a significant impact on growth, development and disease.

NuGEN solutions enable both the characterization of epigenetic changes in the DNA and the elucidation of the impact of those alterations on gene expression.  Our Methyl-Seq Systems allow investigation of direct methylation of DNA via bisulfite sequencing while our Ovation Ultralow Library Systems enable fast library construction for ChIP-Seq, FAIRE-Seq, Hi-C and more. Used in conjunction with our RNA-Seq library systems, researchers can probe both epigenetic modifications of interest and their associated gene expression changes.

Epigenetics as a read-out for inherited traits

Dr. Moreno-Romero's studies focus on the identification of epigenetic modifications that occur during early stages of endosperm development. Methyl-seq libraries were prepared using the Ovation® Ultralow Methyl-Seq Library System and ChIP-seq libraries were prepared with Ovation Ultralow Library System V2 using very small amounts of starting material.

Methylated Biomarkers: A new tool for liquid biopsy

Legendre and Gooden et. al. analyze cell free DNA in breast cancer patients and healthy individuals. Using the Ovation® Ultralow Methyl-Seq Library System, the authors define tumor-specific signatures by identifying 21 hypermethylation hotspots associated with metastatic breast cancer.

ChIPping to an answer: Gene dysregulation in disease development

Dr. Mannini and co-workers investigate the role of the cohesion complex in gene transcription. The scientists use multiple orthogonal methods including ChIP-sequencing with the Ovation® Ultralow Library System V2 to uncover the impact of gene dysregulation as an underlying mechanism for Cornelia de Lange syndrome.