Ovation® Target Capture Module
The Ovation® Target Capture Module provides adaptor-specific blocking reagents to enable Agilent SureSelect target enrichment workflows on libraries generated with the Ovation® Ultralow System V2. This workflow enables efficient target capture with inputs as low as 10 ng with significantly reduced costs and increased throughput via library pooling.
Compatible with libraries generated with the Ovation® Ultralow System V2.
- Enable access to extremely low input and/or FFPE samples
- Pre-hybridization pooling for efficient use of target capture kit
Superior specificity and sensitivity
- High on-target rates even with pre-pooled samples
- Even coverage over entire target region
- Agilent SureSelect Target Enrichment
|Compatible Platform||Illumina HiSeq, MiSeq, NextSeq|
|Starting Material||Libraries generated with Ovation Ultralow System V2|
|Input Amounts||10 pg – 100 ng|
No. The Ovation Target Capture Module is only compatible with the NuGEN sequencing library preparation kits listed in the product User Guide.
Can I use the Ovation Target Capture Module with Ovation Rapid Library Systems?
The yield of the Ovation Rapid Library Systems is not sufficient for the Target Capture methods discussed in the user guide. If the end user has a Target Capture method for which Ovation Rapid Library Systems produce sufficient amounts of library, the Ovation Target Capture Module would be suitable for blocking and subsequent PCR enhancement.
We recommend using high quality DNA with the A260:A280 ratio in ecess of 1.8. Use of DNA samples with lower ratios may result in low library yield.
No. The Amplification Primer Mi provided with the Ovation Target Capture Module is specifically designed for use with the workflow described in the product User Guide. Many systems use the post-capture PCR step to add multipleing barcodes. This is not required with the NuGEN Ovation Target Capture Module workflow.
For research use only. Not for use in diagnostic procedures.