Allegro Targeted Genotyping V2
Allegro Targeted Genotyping V2 provides a fast, scalable, cost-effective approach to perform targeted genotyping-by-sequencing on a wide variety of organisms using next generation sequencing (NGS). With increased sample pooling of 48 samples, Allegro V2 is provided at a 15% lower cost compared to the first generation of Allegro Targeted Genotyping and has been updated to enable fully automatable workflows without manual intervention.
Using the patented Single Primer Enrichment Technology (SPET) for DNA approach to specifically target SNPs of interest, Allegro Targeted Genotyping provides information-rich sequencing data, by capturing a SNP-specific data point for every on-target sequencing read. The result is unparalleled sequencing efficiency, leading to rapid scalability and the lowest cost per data point available.
Allegro Targeted Genotyping combines three core technologies to enable efficient SNP interrogation.
- Enzymatic Fragmentation - Integrated for ease of use and automation
- DimerFree Ligation - eliminates adaptor dimer formation
- SPET - for targeted SNP interrogation
Allegro Targeted Genotyping offers high sample multiplexing capability, ability to interrogate over 100,000 SNPs in a single assay, and flexible design possibilities for targeting new markers.
Product Highlights
Simple, fast, robust automatable workflow
- Simple, single-tube assay that can be completed in less than 24 hours
- Hundreds of barcodes and increased multiplexing enables cost-saving interrogation of SNPs
- Fully automatable on a variety of platforms
Efficient probe interrogation
- Optimal probe placement leads to more efficient sequencing
- Probes are designed on both sides of the SNPs within 100 bp
- Independent probes provide validation of each SNP
Flexible panel designs
- Ability to design panels for any sequenced genome
- Our team has designed for a wide variety of over 60 species and counting
- Expert scientists work with you to generate an optimal design approved by you
For research use only. Not for use in diagnostic procedures.