Celero™ DNA-Seq
DNA-Seq Libraries in 3 Simple Steps
CeleroTM DNA-Seq is an innovative library system designed to help researchers streamline library preparation for Illumina sequencers. Celero features a fast, easy-to-use, addition-only workflow that eliminates post-ligation bead purification. Also included is NuGEN's breakthrough quantitation method, NuQuantTM, that saves both time and cost in measuring library concentration for pooling. With the proven DimerFree technology you can use from 10 ng to 1 μg of intact DNA and greater than 200 ng of FFPE DNA with a single kit without the worry of GC bias.
Why use Celero DNA-Seq?
Celero offers several unique features:
Faster library prep with reduced hands-on time: No adaptor or template dilution and no post- ligation puri cation
One kit, two workflows: PCR and PCR-free can be performed with the same kit, providing flexibility and reducing the number of kits that need to be purchased and stored.
Simplified library pooling: NuQuant provides molarity without the need for costly and time consuming methods.
NuQuant app for Qubit® can be downloaded here on Github.
Product Highlights
Addition-only Workflow
- Celero is an addition-only workflow with 3 master mixes and only 1 bead purification clean-up following library construction.
- Elimination of post-ligation bead purification saves time compared to other methods
High quality libraries
- Streamlined workflow enables generation of high quality libraries
- Reduced base bias
- Provides superior sequencing efficiency of high and low GC organisms
Lower index hopping than other methods
- Significantly lower index hopping observed (0.24%) compared to other current methods (e.g. expected range 0.5-2.0%) using a patterned flow cell
- Unique Dual Indexes available for identifying index hopping events
- Multiplexing up to 384 samples available with Metaplex barcodes
NuQuant: Measuring Molarity in Seconds
- Obtain molar concentrations of individual libraries immediately for multiplex pooling
- A single standard makes reference measurements simple
- More reproducible than qPCR based quantitation methods for sample pooling
- NuQuant app freely available for Qubit® 2.0, 3.0 and 4
Addition-only Workflow
- Celero is an addition-only workflow with 3 master mixes and only 1 bead purification clean-up following library construction.
- Elimination of post-ligation bead purification saves time compared to other methods
High quality libraries
- Streamlined workflow enables generation of high quality libraries
- Reduced base bias
- Provides superior sequencing efficiency of high and low GC organisms
Lower index hopping than other methods
- Significantly lower index hopping observed (0.24%) compared to other current methods (e.g. expected range 0.5-2.0%) using a patterned flow cell
- Unique Dual Indexes available for identifying index hopping events
- Multiplexing up to 384 samples available with Metaplex barcodes
NuQuant: Measuring Molarity in Seconds
- Obtain molar concentrations of individual libraries immediately for multiplex pooling
- A single standard makes reference measurements simple
- More reproducible than qPCR based quantitation methods for sample pooling
- NuQuant app freely available for Qubit® 2.0, 3.0 and 4
For research use only. Not for use in diagnostic procedures.