DNA-Seq library preparation in 3 simple steps
Celero DNA-Seq provides an innovative solution to streamline whole genome sequencing (WGS) library preparation and quantification from mechanically (e.g. Covaris) fragmented DNA. This library prep kit features a fast, easy-to-use, addition-only workflow that eliminates post-ligation bead purification, resulting in faster library preparation and reduced hands-on time.
Celero DNA-Seq is integrated with NuQuant®†, a proprietary library quantification method for efficient and accurate quantitation of Next-Generation Sequencing (NGS) libraries. The NuQuant method eliminates the need for time-consuming or inaccurate library quantification methods like qPCR, fluorometry (e.g. Qubit®) or microfluidic electrophoresis (e.g. Bioanalyzer®) allowing DNA-Seq libraries to be constructed and quantified in less than 3 hours.
When combined with Tecan’s DreamPrep™ NGS automation workflow, Celero DNA-Seq delivers a robust solution for high-throughput WGS and target enrichment applications.
Benefits of Celero DNA-Seq Kit include:
- Works across a broad range of DNA inputs from 10 – 500 ng
- No GC bias provides superior sequencing efficiency
- No adaptor or template dilution and no post-ligation purification step leading to reduced hands-on time
- Flexible multiplexing with 384 dedicated dual index or Unique Dual Index (UDI)* adaptors
- Integrated library quantification to simplify library pooling
NuQuant library quantification app for Qubit® can be downloaded from Github.
Product Highlights
Addition-only Workflow
- Celero is an addition-only workflow with 3 master mixes and only 1 bead purification clean-up following library construction
- Elimination of post-ligation bead purification saves time compared to other methods
High quality libraries for DNA-Seq
- Streamlined workflow enables generation of high quality libraries
- Reduced base bias
- Provides superior sequencing efficiency of high and low GC organisms
Lower index hopping than other methods
- Significantly lower index hopping observed (0.24%) compared to competitor methods (e.g. expected range 0.5-2.0%) using a patterned flow cell
- Unique Dual Indexes available for identifying index hopping events
- Multiplexing up to 384 samples available with Metaplex® barcodes
NuQuant library quantification: Measuring molarity in seconds
- Obtain molar concentrations of individual libraries immediately for multiplex pooling
- A single standard makes reference measurements simple
- More reproducible than qPCR-based library quantitation methods for sample pooling
- NuQuant library quantification app freely available for Qubit® 2.0, 3.0 and 4
† NuQuant is enabled for use with Part Nos. 0360-24, 0360A-A01, 0360B-A01, 0360C-A01, 0360D-A01, and 0360A-UDI. For questions or concerns please contact Tecan NGS Technical Support.
For research use only. Not for use in diagnostic procedures.