Celero™ DNA-Seq Library Preparation Kit
DNA-Seq Libraries in 3 Simple Steps
The Celero DNA-Seq library preparation kit is an innovative system designed to help researchers streamline library preparation for Illumina sequencers. Celero features a fast, easy-to-use, addition-only workflow that eliminates post-ligation bead purification. Also included is NuGEN's breakthrough library quantitation method, NuQuant®, that saves both time and cost in measuring library concentration for pooling. With the proven DimerFree™ technology you can use from 10 ng to 1 μg of intact DNA and greater than 200 ng of FFPE DNA with a single kit without the worry of GC bias.
Celero DNA-Seq library preparation kit offers several unique benefits:
Short, simple workflow: NGS library preparation in 3 steps with reduced hands-on time, no adaptor or template dilution or post-ligation purification.
No adaptor dimers: get more informative reads compared without multiple bead clean-ups.
Flexible multiplexing: dedicated dual index or Unique Dual Index adaptors for each reaction included with the kit.
Flexible workflow: PCR and PCR-free workflows using the same kit, providing flexibility and reducing the number of kits that need to be purchased and stored.
Integrated library quantification: quickly determine library molarity without the need for costly and time-consuming processes.
Automatable: enabled on multiple liquid-handling platforms
NuQuant library quantification app for Qubit® can be downloaded from Github.
Product Highlights
Addition-only Workflow
- Celero is an addition-only workflow with 3 master mixes and only 1 bead purification clean-up following library construction
- Elimination of post-ligation bead purification saves time compared to other methods
High quality libraries for DNA-Seq
- Streamlined workflow enables generation of high quality libraries
- Reduced base bias
- Provides superior sequencing efficiency of high and low GC organisms
Lower index hopping than other methods
- Significantly lower index hopping observed (0.24%) compared to other current methods (e.g. expected range 0.5-2.0%) using a patterned flow cell
- Unique Dual Indexes available for identifying index hopping events
- Multiplexing up to 384 samples available with Metaplex barcodes
NuQuant library quantification: Measuring molarity in seconds
- Obtain molar concentrations of individual libraries immediately for multiplex pooling
- A single standard makes reference measurements simple
- More reproducible than qPCR-based library quantitation methods for sample pooling
- NuQuant library quantification app freely available for Qubit® 2.0, 3.0 and 4
Addition-only Workflow
- Celero is an addition-only workflow with 3 master mixes and only 1 bead purification clean-up following library construction
- Elimination of post-ligation bead purification saves time compared to other methods
High quality libraries for DNA-Seq
- Streamlined workflow enables generation of high quality libraries
- Reduced base bias
- Provides superior sequencing efficiency of high and low GC organisms
Lower index hopping than other methods
- Significantly lower index hopping observed (0.24%) compared to other current methods (e.g. expected range 0.5-2.0%) using a patterned flow cell
- Unique Dual Indexes available for identifying index hopping events
- Multiplexing up to 384 samples available with Metaplex barcodes
NuQuant library quantification: Measuring molarity in seconds
- Obtain molar concentrations of individual libraries immediately for multiplex pooling
- A single standard makes reference measurements simple
- More reproducible than qPCR-based library quantitation methods for sample pooling
- NuQuant library quantification app freely available for Qubit® 2.0, 3.0 and 4
For research use only. Not for use in diagnostic procedures.