Product Notice (Effective Oct. 1, 2020)
The products known as Celero PCR Workflow with Enzymatic Fragmentation DNA-Seq kit (Part No. 9363) will be known as Celero EZ DNA-Seq (Part Nos. 0568, 0569).
Product performance and kit configurations have not changed. Contact your Account Executive or Tech Support at techserv-gn@tecan.com for more details.
DNA-Seq with enzymatic fragmentation in 3 simple steps
Celero EZ DNA-Seq provides an innovative solution to streamline whole genome sequencing (WGS) library preparation and quantification. This library prep kit features a fast, easy-to-use, addition-only workflow that eliminates post-ligation bead purification, resulting in faster library preparation and reduced hands-on time.
Celero EZ DNA-Seq features our enzymatic fragmentation technology that is simple, robust and consistent for fragmentation of genomic DNA into homologous sized fragments. This technology provides greatly improved fragmentation without optimization, outperforming standard DNA-Seq kits for a broad range of sample types and concentrations to provide the best possible starting material for your sequencing protocol.
DNA libraries from Celero EZ provide higher yields and better uniformity of coverage, ideal for downstream target enrichment applications like whole exome sequencing. When combined with Tecan’s DreamPrep™ NGS automation workflow, Celero EZ DNA-seq delivers a robust solution for high throughput WGS and target enrichment applications.
- Broad input range from 10 ng – 500 ng
- Tunable, optimization-free enzymatic fragmentation for all standard sample types
- No adaptor or template dilution and no post-ligation purification step leading to reduced hands-on time
- Flexible multiplexing with 384 dedicated dual index or Unique Dual Index (UDI)† adaptors
- Integrated library quantification to simplify library pooling
This library preparation kit is integrated with NuQuant®†, a proprietary library quantification method for efficient and accurate quantitation of Next-Generation Sequencing (NGS) libraries. The NuQuant method eliminates the need for time-consuming or inaccurate library quantification methods like qPCR, fluorometry (e.g. Qubit®) or microfluidic electrophoresis (e.g. Bioanalyzer®) allowing DNA-Seq libraries to be constructed and quantified in less than 3 hours.
Product Highlights
A simple and robust solution for DNA-Seq library preparation
- A single workflow for DNA-Seq library preparation with integrated library quantification method
- One bead clean-up step following PCR
- Flexible and robust fragmentation to generate insert sized from 200-500 bp
- Integrated NuQuant library quantification for accurate NGS library molarity quantification
- Eliminate the need of time-consuming qPCR for library quantification
- Compatible with standard fluorometers such as the Qubit or standard plate readers
Whole exome sequencing with Celero EZ DNA-Seq
- Enable more accurate variant calling - Celero EZ DNA-Seq library preparation kit consistently maintains greater than 98% target coverage
- Even depth of coverage of target sequences compared to competitor
- Greater uniformity of coverage reduces the number of reads required, enabling further cost savings
† NuQuant is enabled for use with Part Nos. 0568 and 0569A-A01 only. For questions or concerns please contact Tecan NGS Technical support.
For research use only. Not for use in diagnostic procedures.