simple clean science

NuGEN's Methyl-Seq product line is comprised of library preparation and bisulfite conversion tools for a broad range of applications for NGS, methylation arrays, and more. Now, with the integration of TrueMethyl® oxBS chemistry, gain new insight into epigenetic modifications with the ability to interrogate both 5mC and 5hmC.


The products feature NuGEN's DimerFree ligation technology to enable streamlined, simple workflows for a broad range of input quantities and sample types. The Ovation® Ultralow Methyl-Seq library system provides a comprehensive approach to DNA methylation detection across the entire genome from many different sample types. The Ovation® RRBS Methyl-Seq system enables a focused and cost-efficient approach to assessing methylation state of regions with high CpG density through a reduced-representation method.


  • Workflows for a wide range of samples, including intact genomic DNA, FFPE DNA, cell-free DNA, ChIP DNA, and more
  • DimerFree ligation technology to enable a wide range of sample inputs down to 10 ng
  • TrueMethyl oxBS chemistry for thorough conversion and accurate interrogation of the genome
  • Barcoding options up to 96 samples to make the best use of the latest sequencers
  • Inclusion of diversity adaptors eliminates the need for PhiX spike-in, saving sequencing costs
  • Systems work hand-in-hand with DNA-Seq and RNA-Seq systems to interrogation all aspects of epigenetic regulation