Rapid EZ DNA-Seq Library Preparation Kit
Rapid EZ DNA-Seq library preparation kit is an end-to-end solution for generating PCR-Free NGS libraries in 2.5 hours. This kit provides a simple add and incubate workflow with optimization-free, robust enzymatic fragmentation and DimerFree® library construction. This kit includes all reagents needed for library construction including Unique Dual Index (UDI) adaptors for each reaction to enable detection of "index hopping" and flexible multiplexing.
This NGS library preparation workflow is suitable for a wide range of sequencing applications. The PCR-Free workflow is particularly useful for applications sensitive to PCR bias or PCR artifacts such as sequencing of GC or AT rich regions of the human genome, or microbiome and prokaryotic samples.
- Single kit provides all the reagents necessary for PCR-free NGS library preparation including per-plated adaptors
- Short, add and incubate workflow that can be completed in 2.5 hours
- Flexible and robust enzymatic fragmentation to generate insert sizes from 200 - 500 bp without optimization
- Up to 96 Unique Dual Index (UDI) adaptors standard for flexible multiplexing
- Eliminate adaptor dimers without adaptor titration regardless of sample input using the DimerFree technology
- PCR-Free workflow enables high quality data for any sample type, including microbial genomes with a broad range of GC content
Product Highlights
Simple and robust enzymatic PCR-free solution for DNA-Seq library preparation
- Add and incubate workflow without PCR amplification, eliminating PCR bias
- Flexible and robust fragmentation to generate insert sizes from 200-500 bp without optimization
- No sample conditioning or adaptor dilution steps necessary
Robust enzymatic fragmentation consistent across different inputs and replicates
- No optimization needed
- Robust fragmentation consistent across different sample types, inputs and GC content
For research use only. Not for use in diagnostic procedures.