As next-generation sequencing technology advances, the need for highly efficient, unbiased library generation for a wide variety of inputs has grown. High-throughput sequencing operations need library preparation workflows that optimize many factors, such as sample preparation and processing efficiency without compromising the quality of downstream bioinformatics analysis.
Rapid EZ DNA-Seq library preparation kit can meet these needs as it provides a fast and simple workflow with optimization-free, robust enzymatic fragmentation, and DimerFree® library construction.
The PCR-free workflow in Rapid EZ library preparation kit contains only one bead clean-up step, and supports samples that are sensitive to PCR bias or PCR artifacts such as the sequencing of GC- or AT-rich regions of the human genome, or microbiome and prokaryotic samples.
Benefits of Rapid EZ DNA-Seq library preparation kit:
- Short add and incubate workflow that can be completed in 2.5 hours
- Detect index hopping with unique dual indexes (UDI) adaptors
- Eliminate adaptor dimers without adaptor titration regardless of sample input using the DimerFree technology
Product Highlights
Simple and robust enzymatic PCR-free solution for DNA-Seq library preparation
- Add and incubate workflow without PCR amplification, eliminating PCR bias
- Flexible and robust fragmentation to generate insert sizes from 200-500 bp without optimization
- No sample conditioning or adaptor dilution steps necessary
Robust enzymatic fragmentation consistent across different inputs and replicates
- Flexible and robust fragmentation to generate insert sizes from 200-500 bp without optimization
- Fragmentation consistent across different sample types, inputs and GC content
For research use only. Not for use in diagnostic procedures.