What is RNA-Seq?
RNA sequencing (RNA-Seq) is a powerful technique that can examine the presence and quantity of RNA in samples by using next-generation sequencing (NGS). This tool is able to provide insight into the transcriptome of the cell by using high-throughput sequencing methods. It can facilitate research in gene expression, novel genes (or transcripts) discovery, splicing and mutation analysis.
Benefits of RNA-Seq
Compared to gene expression microarrays and qPCR, RNA-Seq offers a broad range of advantages.
First sequencing-based method that allows the intact transcriptome to be surveyed in a very high-throughput and quantitative manner
Ability to simultaneously map transcribed regions and gene expression
High range of gene expression detection level (>8,000-fold)
Applicable for a wide range of RNA samples with broad range of input amount, including access to degraded samples such as FFPE
RNA-Seq is useful to apply in many scientific areas including medical, agricultural, food, and biological sciences. RNA-Seq can be used for differential gene expression, variation detection, allele-specific expression, systems biology, and single cell analysis.
RNA-Seq applications with Tecan kits:
Tecan's RNA-Seq library preparation kits are used globally by researchers in different scientific fields, with multiple peer-reviewed publications.
Tecan's RNA-Seq library preparation kits provide the first complete solution, combining library preparation, targeted transcript depletion (AnyDeplete®), library quantification (NuQuant®) and DimerFree® adaptor ligation in a fully integrated workflow.
Multiplex a large number of samples
Customized transcript depletion for any organism
Save time and resources on library quantification with integrated QC technology
Detect rare transcripts or pathogens from challenging samples using our unique amplification technology
Use the RNA-Seq selection guide to find the right kit for your application.