What is RNA-Seq?

RNA sequencing (RNA-Seq) is a powerful technique that can examine the presence and quantity of RNA in samples by using next-generation sequencing (NGS). This tool is able to provide insight into the transcriptome of the cell by using high-throughput sequencing methods. It can facilitate research in gene expression, novel genes (or transcripts) discovery, splicing and mutation analysis.

 

Benefits of RNA-Seq

Compared to gene expression microarrays and qPCR, RNA-Seq offers a broad range of advantages.

  • First sequencing-based method that allows the intact transcriptome to be surveyed in a very high-throughput and quantitative manner

  • Ability to simultaneously map transcribed regions and gene expression

  • High range of gene expression detection level (>8,000-fold)

  • Applicable for a wide range of RNA samples with broad range of input amount, including access to degraded samples such as FFPE

 

RNA-Seq is useful to apply in many scientific areas including medical, agricultural, food, and biological sciences. RNA-Seq can be used for differential gene expression, variation detection, allele-specific expression, systems biology, and single cell analysis.

RNA-Seq applications with Tecan kits:

Tecan's RNA-Seq library preparation kits are used globally by researchers in different scientific fields, with multiple peer-reviewed publications.

Tecan's RNA-Seq library preparation kits provide the first complete solution, combining library preparation, targeted transcript depletion (AnyDeplete®), library quantification (NuQuant®) and DimerFree® adaptor ligation in a fully integrated workflow.

  • Multiplex a large number of samples

  • Customized transcript depletion for any organism

  • Save time and resources on library quantification with integrated QC technology

  • Detect rare transcripts or pathogens from challenging samples using our unique amplification technology

Use the RNA-Seq selection guide to find the right kit for your application.