Targeted Genotyping

Tecan's Allegro® Targeted Genotyping provides a fast, scalable, cost-effective approach for targeted genotyping-by-sequencing on a wide variety of organisms using NGS.


This workflow combines three core technologies to enable efficient SNP interrogation:

  • Enzymatic fragmentation - integrated for ease of use and automation
  • DimerFree® ligation - eliminates adaptor dimer formation
  • Single Primer Enrichment Technology (SPET) - for targeted SNP interrogation


Allegro offers high sample multiplexing capabilities, the ability to interrogate over 100,000 SNPs in a single assay, and flexible design possibilities for targeting new markers.

  • Simple, single-tube assay that can be completed in less than 24 hours
  • Optimal probe placement leads to more efficient sequencing
  • Independent probes provide validation of each SNP
  • Ability to multiplex up to 3,072 libraries with the Allegro Metaplex Module
  • Flexible panel designs for any sequenced genome