Technology

Product Details

High-throughput genomic technologies such as next generation sequencing and microarray enable researchers to survey thousands of genes or transcripts simultaneously, but they are not without their own unique challenges.

NuGEN offers NGS and genomic sample preparation products featuring technologies that address common limitations and maximize informative sequencing and gene expression data from any sample, from high to low quality and microgram to picogram inputs.

Find out more about our core technologies below:

Single Primer Enrichment Technology (SPET) for DNA : Directly enrich regions of DNA for targeted re-sequencing, SNP (SNV) detection, CNV analysis, viral detection, and more.
Single Primer Enrichment Technology (SPET) for RNA : Directly enrich cDNA targets for gene fusion detection and discovery.
Single Primer Isothermal Amplification (SPIA) : Robust cDNA amplification for multiple workflows.
AnyDeplete (formerly InDA-C) : Stranded RNA-Seq library preparation with targeted transcript depletion.

Single Primer Enrichment Technology (SPET) for DNA

Probes customizable to any DNA target are mixed into samples.
Probes are extended and adaptors are added to prepare the library.
Only targeted sequences are present in the final library.

Single Primer Enrichment Technology (SPET) for DNA is a customizable solution for targeted sequencing. SPET probes interrogate both strands of the target to enable CNV analysis, robust detection of SNPs (SNVs) and viruses, and re-sequencing.

High quality or FFPE input
Flexible customization from one to thousands of targets
Molecular tag for accurate determination of unique molecules
Superior alignment, on-target and coverage metrics

Find this technology in:

Ovation Cancer Panel 2.0

Ovation Custom Target Enrichment System

Single Primer Enrichment Technology (SPET) for RNA

cDNA is prepared with an included cDNA module.
Probes customizable to any fusion are mixed into samples.
Probes are extended into neighboring exons and fusions to prepare the library.
Only targeted exons and fusions are present in the final library.

Single Primer Enrichment Technology (SPET) for RNA is a customizable solution for gene fusion and translocation detection. Probes extend into neighboring exons or fusions during library construction to identify both known and novel targets.

High quality or FFPE input
Flexible customization from one to thousands of targets
Molecular tag for accurate determination of unique molecules
Superior alignment, on-target and coverage metrics

Find this technology in:

Ovation Cancer Panel 2.0

Ovation Custom Target Enrichment System

Single Primer Isothermal Amplification (SPIA)

Single primer isothermal amplification (SPIA) is a highly reproducible method of isothermal amplification that preserves the stoichiometry of the input material. Our SPIA systems generate cDNA for a range of samples and workflows:

Low input RNA-seq and gene expression analysis with microarray and qPCR
Pre-amplification for qPCR
Whole transcriptome amplification
Works with RNA from all sample types–Liquid biopsies, FFPE, LCM, cell lines, tissues
Automated workflows available

Find this technology in:

Ovation RNA-Seq System V2

Ovation RNA-Seq FFPE System

Trio RNA-Seq

AnyDeplete (formerly InDA-C)

Whole transcriptome RNA-Seq libraries contain abundant sequences, such as those derived from rRNA (shown in red), which offer limited biological information for many experiments.
After library construction, probes that target unwanted sequences (AnyDeplete probes) are mixed into the sample. The probes are customizable to any abundant transcript, and because they are targeted to specific transcripts, there are minimal off-target effects.
The AnyDeplete (formerly known as InDA-C) probes facilitate degradation of unwanted sequences.
The final amplified libraries provide an enrichment in potential sequences of interest due to ~95% (Universal) or >80% (SoLo) depletion of unwanted transcripts.

AnyDeplete (formerly InDA-C) is a technology for targeted depletion of abundant transcripts.

Customizable to any transcript, any organism
Post library depletion avoids off-target mRNA cross-hybridization events that can introduce unwanted bias
Maximize informative sequencing reads in whole transcriptome data

Find this technology in:

Ovation Universal RNA-Seq System

Ovation SoLo RNA-Seq System

Trio RNA-Seq

Universal Plus mRNA-Seq