Universal Plus mRNA-Seq Library Preparation Kit with NuQuant

Product Highlights

NuQuant Library Quantification Method: Accurately quantify molar concentrations of NGS libraries

NuQuant Library Quantification is a proprietary method by which fluorescent labels are incorporated into the library molecules during the library preparation process. Each library molecule has an equivalent number of labels incorporated, regardless of the size of the library fragment, resulting in direct measurement of the molar concentration using standard fluorometers. NGS library quantification with NuQuant is accurate, easy to use and is integrated with the Universal mRNA-Seq with NuQuant library preparation kit.

  • Accurately measure molar library concentration without the need for library size analysis (e.g. Bioanalyzer) unlike Qubit mass measurement or qPCR
  • Quantify only sequenceable molecules unlike Qubit and Bioanalyzer
  • Eliminate the need of time consuming qPCR for library quantification
  • Compatible with standard fluorometers such as the Qubit or standard plate readers

Reproducible mRNA-Seq library preparation and sequencing results

  • Simple one-day workflow from total RNA to quantified RNA-Seq libraries for Illumina sequencers
  • Consistent results across a wide range of inputs from 10 ng to 1 µg of total RNA
  • Uniform coverage across the entire transcript

Remove unwanted reads with AnyDeplete

  • Streamlined workflow to eliminate abundant unwanted reads (i.e. globin, mitochondrial transcripts, rubisco, etc.)
  • Simple customization of AnyDeplete probe design to optimize your experiment
  • Simplify data analysis and reduce sequencing costs

For research use only. Not for use in diagnostic procedures.