Spotlight on Science
Mining the Post-Mortem Human Brain for Neurodegenerative Markers

Dr. Steve Sheardown is the Head of Molecular Biology and Cell Engineering at Cerevance. Cerevance is focused on answering fundamental questions about the complexity of the central nervous system and its diseases. Dr. Sheardown and colleagues are especially interested in identifying changes in gene expression associated with neurodegenerative disorders where effective disease management or therapeutics are not available. Learn more about Dr. Sheardown's research and how Trio RNA-Seq enabled his team to investigate the impact of disease states on individual neuronal cell types using a high-throughput approach.

Mapping Hydroxymethylation Patterns in Neurodegenerative Disease

Dr. Mill is a Professor of Epigenetics at the University of Exeter Medical School in the United Kingdom where he is interested in understanding the causes and consequences of genomic variation in the brain, and the role this plays in neuropsychiatric and neurodegenerative disease. To better understand how DNA methylation and hydroxymethylation play a role in neurobiology, Dr. Mills' research has taken advantage of the TrueMethyl oxBS technology to quantify the 'true' levels these DNA modifications and to better determine how gene regulation is affected in specific neural cell populations.

Transcriptional profiling of pain-transmitting neurons in the spinal dorsal horn

Mr. Chamessian is a doctoral candidate at the Duke University School of Medicine where he is investigating the pain-transmitting neurons in the spinal cord to understand the causes and mechanisms of chronic pain. To answer these questions, Mr. Chamessian has been using Ovation® SoLo RNA-Seq to identify distinct cell populations and determine the genes that govern the function of these cells under normal and pathological conditions.

Emerging Disease Surveillance and Diagnostic Method Development for Veterinary Medicine

Dr. Knutson is a computational biologist in the Molecular Development Lab at the University of Minnesota’s Veterinary Diagnostics Lab (VDL) where he is involved in research projects to monitor new and existing pathogens infecting animals from agricultural or the wild. Recent work by Dr. Knutson in the VDL have been enabled by the powerful new Trio RNA-Seq library kit.

Deciphering Key Components of Human Hair Follicle Regeneration

Dr. Weber's research focuses on identifying the transcriptional regulators which are responsible for defining the dermal hair follicle stem cell with the goal of developing a renewable source of hair follicle stem cells for transplantation or full follicle development in vitro. The Ovation® RNA-Seq System V2 was used to prepare cDNA for sequencing libraries from very low inputs of starting material.

Understanding Epigenetic Mechanisms in Endosperm Development

Dr. Moreno-Romero's studies focus on the identification of  epigenetic modifications that occur during early stages of endosperm development. DNA methylation libraries were prepared using Ovation® Ultralow Methyl-Seq Library Systems and ChIP libraries were prepared with Ovation® Ultralow Library Systems V2 using very small amounts of starting material.

Validation of Workflow and Reagents for RNA-Seq Using Whole Blood

Dr. Brooks designed and led a multi-site study for the clinical validation of an end-to-end workflow for the collection, storage, transport and preparation of human whole blood samples for RNA-Seq. The study integrated products from PreAnalytiX GmbH and the Ovation® Human Blood RNA-Seq System in a collaborative effort with RUCDR Infinite Biologics at Rutgers University to measure the system’s efficacy for transcriptome profiling with clinical samples. At the conclusion of the study it was determined that the integrated workflow ensures reproducible, accurate and sensitive results in RNA-Seq of whole blood, enabling gene expression from total RNA thereby allowing researchers to study both protein coding and regulatory transcripts from human whole blood.

Identifying Mutations that Impact Human Aging and Disease

Dr. Tranah is a Professor at the California Pacific Medical Center (CPMC) Research Institute and an Adjunct Professor in the Department of Epidemiology and Biostatistics at the University of California, San Francisco. Dr. Tranah’s current research program is focused on identifying inherited and acquired genetic factors that impact aging and disease with the goal of revolutionizing risk assessment and identifying widely applicable and inexpensive genomic tests that identify persons who would benefit from specific pharmacologic and behavioral treatments to prevent disability and disease.