Single cell RNA-seq for whole transcriptome sequencing

Are you worried about your current single cell sequencing solution disappearing?

There is a lot of confusion in the single cell, ultra-low input RNA sequencing market right now. Some manufacturers are impacted by lawsuits which may restrict their ability to supply kits. As a researcher it’s easy to be worried by all this upheaval. The good news is that Tecan’s Ovation SoLo RNA-Seq Kit is very much here to stay. The SoLo RNA-Seq kit is the perfect library prep choice for low input degraded samples down to just 10 pg of starting RNA. SoLo RNA-Seq is compatible with whole transcriptome sequencing, exosomal RNA sequencing, single cell sequencing, low quality and degraded samples, and any application where input material is limited.

The simplest, most robust solution for ultra-low input RNA Sequencing

Not only is it here to stay but the Tecan Ovation SoLo RNA-Seq Kit is the only available ultra-low RNA library preparation kit that includes all of the Tecan proprietary workflow enhancements for a robust simple workflow.

Ovation SoLo RNA-Seq Kit at a glance:

Feature

Ultra low input

Compatible with cell lysate

Works with degraded samples

Removes unwanted transcripts

Includes molecular tags

Preplated adaptors

Benefit

Yes -

Start with as

little as 10 pg

Yes -

No RNA isolation

Yes -

Including FFPE

and other degraded

sample types

Yes -

AnyDeplete included

Yes -

Detect and

eliminate

duplicates

Yes -

No adaptor

titration

required

The most streamlined workflow for RNA Sequencing

The Ovation SoLo RNA-Seq Kit has the simplest, robust and most user-friendly workflow of any ultra-low RNA prep kit. This kit features Tecan’s proprietary AnyDeplete technology to remove unwanted rRNA transcripts after library construction. Unlike up-front rRNA depletion methods such as Ribo-Zero, AnyDeplete enables  much lower RNA input levels (down to 10 pg) while still offering effective elimination of unwanted reads prior to sequencing. SoLo RNA-Seq with AnyDeplete increases the dynamic range that can be studied, reduces sequencing costs and simplifies data analysis.

Figure 1: Ovation SoLo RNA-Seq is a complete workflow enabling whole transcriptome analysis from ultra low inputs.

High performance at ultra-low input levels

Ovation SoLo RNA-Seq System is a complete end-to-end sample to library preparation solution for whole transcriptome analysis starting with ultra-low starting material. This makes SoLo RNA-Seq the kit of choice for:

Results from samples with starting concentrations ranging from 1 ng to 10 pg show high correlation. SoLo RNA-Seq yields the same sequence data across a broad range of samples and down to extremely low (single cell) input levels, providing a high confidence in your sequence data.

Figure 2: High correlation between technical replicates from inputs as low as 10 pg of total RNA giving you confidence in your data.

Benefits summary

  • Whole transcriptome RNA-Seq analysis from ultra-low starting material
  • 10 pg to 10 ng of isolated RNA or cell lysate from 1-500 cells
  • Fully integrated user friendly workflow
  • Direct integration with cell lysis protocols
  • Efficient stranded library preparation without adaptor titration
  • Pre-plated adaptors with unique barcodes included for every sample
  • Integrated molecular tag for accurate detection of PCR duplicates
  • Customizable transcript depletion post-library preparation 

Ordering information

Product

Part Number

Reaction Size

Ovation SoLo RNA-Seq, Human AnyDeplete

0500

32, 96

Ovation SoLo RNA-Seq, Mouse AnyDeplete

0501

32, 96

Ovation SoLo RNA-Seq, Drosophila AnyDeplete

0502

32, 96

Ovation SoLo RNA-Seq, Custom AnyDeplete

Contact your account executive

 

AnyDeplete probe sets can be customized to any transcript from any organism. For custom probe sets, contact your account executive or request a quote via our website.

 

For Research Use Only. Not for use in diagnostic procedures.